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. 1997 Sep 15;186(6):921–929. doi: 10.1084/jem.186.6.921

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TCR and immunoglobulin gene rearrangement in Ku70⁻ ^/− mice. (A) Recombination of V558L, V7183 to DJ_H, and D_H to J_H gene segments (26). 100 ng DNA was used for Ku70⁻ ^/− (lanes 7 and 8), Ku80⁻ ^/− (lanes 1–3), and scid mice (lanes 4–6), and 1, 10, and 100 ng for wild-type (WT) mice (lanes 9– 11). For IVS controls, DNA was diluted 100-fold before PCR. (B) PCR analysis of TCR gene rearrangements. Thymus DNA was assayed for recombination of V_β8-J_β2 and Dδ2 to Jδ1 rearrangements (20, 27, 28). 100 ng DNA was used for Ku70⁻ ^/− (lanes 2 and 7), Ku80⁻ ^/− (lane 1), and Ku70⁺ ^/− mice (lane 7), and 1, 10, and 100 ng for wild-type mice (lanes 4–6). Controls include a 1-kb germline interval amplified in the Dδ2 to Jδ1 intervening region (germline), and a nonrecombining segment of the Ig locus between J_H and C_H1 (not shown). The same thymus DNA samples were examined for V_β8-J_β2 and Dδ2 to Jδ1 recombination. Abbreviations: DJ_H, D_H to J_H rearrangements; V₇₁₈₃J_H and V_558LJ_H, V7183 and V558L to DJ_H rearrangements (26); V_β8J_β2.1 to V_β8J_β2.6, V_β8 to DJ_β2 rearrangements (28); germline, unrecombined DNA from the Dδ2 to Jδ1 interval; Dδ ₂ Jδ ₁, Dδ2 to Jδ1 rearrangements (20, 27); IVS, nonrecombining segment of the Ig locus between J_H and C_H1 (26). Multiple lanes underneath each genotype label (Ku70⁻ ^/−, Ku80⁻ ^/−, and SCID) represent different individual animals.

TCR and immunoglobulin gene rearrangement in Ku70⁻ ^/− mice. (A) Recombination of V558L, V7183 to DJ_H, and D_H to J_H gene segments (26). 100 ng DNA was used for Ku70⁻ ^/− (lanes 7 and 8), Ku80⁻ ^/− (lanes 1–3), and scid mice (lanes 4–6), and 1, 10, and 100 ng for wild-type (WT) mice (lanes 9– 11). For IVS controls, DNA was diluted 100-fold before PCR. (B) PCR analysis of TCR gene rearrangements. Thymus DNA was assayed for recombination of V_β8-J_β2 and Dδ2 to Jδ1 rearrangements (20, 27, 28). 100 ng DNA was used for Ku70⁻ ^/− (lanes 2 and 7), Ku80⁻ ^/− (lane 1), and Ku70⁺ ^/− mice (lane 7), and 1, 10, and 100 ng for wild-type mice (lanes 4–6). Controls include a 1-kb germline interval amplified in the Dδ2 to Jδ1 intervening region (germline), and a nonrecombining segment of the Ig locus between J_H and C_H1 (not shown). The same thymus DNA samples were examined for V_β8-J_β2 and Dδ2 to Jδ1 recombination. Abbreviations: DJ_H, D_H to J_H rearrangements; V₇₁₈₃J_H and V_558LJ_H, V7183 and V558L to DJ_H rearrangements (26); V_β8J_β2.1 to V_β8J_β2.6, V_β8 to DJ_β2 rearrangements (28); germline, unrecombined DNA from the Dδ2 to Jδ1 interval; Dδ ₂ Jδ ₁, Dδ2 to Jδ1 rearrangements (20, 27); IVS, nonrecombining segment of the Ig locus between J_H and C_H1 (26). Multiple lanes underneath each genotype label (Ku70⁻ ^/−, Ku80⁻ ^/−, and SCID) represent different individual animals.