Figure 5.

Ontogeny of TCR-γ transcripts. Identification of Vγ1-Jγ-Cγ, Vγ2-Jγ-Cγ, and Vγ3-Jγ-Cγ transcripts by PCR amplification. Actin expression was used to standardize cDNA amounts and to allow semiquantitative analysis. Templates were cDNA from thymus at various stages of development. After 32 cycles of amplification, PCR products were electrophoresed, stained with ethidiumbromide, and photographed. (A) Ontogeny of the different Vγ transcripts in chicken embryos. (B) Comparative analysis of the different Vγ transcripts in the three developmental waves of thymocytes. Thymocytes derived from the first, second, and third wave of precursors were from E13 embryos, 1-d-old chicks (day 22), and 1-wk-old chicks (day 28), respectively. First, second, and third wave thymocytes were obtained from multiple embryos.