Table 3.
Influence of I-Ab,I-Ak, I-Aq, and H2nb1 MHC Class II Molecules on 4.1 Thymocyte Development
| Percentage of cells* | Ratios* | IDDM‡ (n) | Insulitis‡ score (n) | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| n | Organ | CD4+CD8−(Vβ11+) | CD4+CD8+ | CD4−CD8− | CD4−CD8+ | CD4+/ DN | CD4+/CD8+ | |||||||||||||
| x ± SD | x ± SD | |||||||||||||||||||
| (NOD × B6) F1 | 29 | T | 15 ± 5a (67 ± 7) | 53 ± 10 | 27 ± 9 | 4 ± 3 | 0.6 ± 0.2s | – | 0 (10)ae | 0.42 ± 0.81 (10)ak | ||||||||||
| S | 12 ± 6 (78 ± 12) | – | 84 ± 8 | 4 ± 2 | – | 2.9 ± 1.5y | ||||||||||||||
| (NOD × I-Ab−B6) F1 | 9 | T | 36 ± 5b (93 ± 3) | 37 ± 9 | 23 ± 4o | 4 ± 1 | 1.6 ± 0.2t | – | 0 (15)af | 1.59 ± 0.45 (10)al | ||||||||||
| S | 12 ± 6c (86 ± 11) | – | 86 ± 7 | 2 ± 1 | – | 4.6 ± 1.4z | ||||||||||||||
| NOD | 9 | T | 30 ± 7d (94 ± 2)e | 53 ± 9 | 13 ± 4p | 4 ± 2 | 2.2 ± 0.6u | – | 30 (40)ag | 3.74 ± 0.15 (4)am | ||||||||||
| S | 24 ± 4f (88 ± 4)g | – | 72 ± 5 | 4 ± 1 | – | 8.7 ± 2.9aa | ||||||||||||||
| NOD.H-2g7/I-Ak | 7 | T | 25 ± 3 (93 ± 1) | 49 ± 5 | 21 ± 3q | 5 ± 1 | 1.2 ± 0.2v | – | 0 (9)ah | 2.2 ± 0.8 (7)an | ||||||||||
| S | 6 ± 1h (90 ± 1) | – | 92 ± 1 | 2 ± 1 | – | 2.9 ± 0.3ab | ||||||||||||||
| NOD.H-2g7/q | 7 | T | 19 ± 3i (88 ± 3) | 59 ± 5 | 19 ± 3 | 3 ± 1 | 1.0 ± 0.2w | – | 0 (7)ai | 0.7 ± 0.4 (7)ao | ||||||||||
| S | 11 ± 8j (87 ± 4) | – | 86 ± 10 | 3 ± 2 | – | 2.9 ± 1.4ac | ||||||||||||||
| NOD.H-2g7/nb1 | 11 | T | 11 ± 3k (37 ± 9)l | 52 ± 7 | 34 ± 5r | 3 ± 1 | 0.3 ± 0.1x | – | 0 (11)aj | 0.1 ± 0.1 (7)ap | ||||||||||
| S | 6 ± 2m (51 ± 16)n | – | 91 ± 4 | 2 ± 1 | – | 2.9 ± 0.4ad | ||||||||||||||
Mice were studied at 3–5 (
) or at 10 wk (
) of age. Flow cytometry was done as indicated in the legend to Fig. 1. DN, double-negative cells (CD4−CD8−); T, thymus; S, spleen. Statistics (Mann-Whitney U test): CD4+CD8−: a versus b, P <0.0001; c versus f, P <0.003; h versus f, P <0.0009; i versus d, P <0.0001; j versus f, P <0.005; k versus d, P <0.0002; m versus f, P <0.0002; CD4+CD8−Vβ11+: 1 versus e, P <0.0002; n versus g, P <0.0002; CD4−CD8−: o versus p, P <0.03; q versus p, P <0.015; r versus p, P <0.0002; CD4+/DN: s versus t, P <0.0001; u versus v, P <0.03; w versus u, P <0.002; x versus u, P <0.0002; CD4+/CD8+: y versus z, P <0.015; z versus aa, P <0.002; ab versus aa, P <0.0009; ac versus aa, P <0.0002; ad versus aa, P <0.0002; IDDM: ad,ae,ah,ai,aj versus ag, P <0.0001; Insulitis Score: al versus ak, P <0.0006; ak versus am, P <0.0005; al versus am, P <0.005; an versus am, P <0.03; ao versus am, P <0.008; ap versus am, P <0.006.