Table 5.
Inhibition of Activation of AP24 by Agents That Suppress Apoptosis
| Experiment | Cell treatment* | AP24‡ | AP24 nuclear activity | Apoptotic cells | ||||
|---|---|---|---|---|---|---|---|---|
| nmol/mg/h | U/ml | % | ||||||
| 1 | — | 362 | <2 | ND | ||||
| TNF 2 ng/ml | 1395 | 8 | ND | |||||
| TNF + cpDEVD-CHO 2 μM | 777 | 2 | ND | |||||
| TNF + DK120 5 μM | 805 | 3 | ND | |||||
| 2 | — | 139 | ND | 0 | ||||
| TNF 5 ng/ml | 722 | ND | 48 | |||||
| TNF + VAD-cmk 2.5 μM | 389 | ND | 29 | |||||
| 3 | — | 494 | <2 | 0 | ||||
| UV light 0.06 J/cm2 | 947 | 8 | 48 | |||||
| UV light + TPCK 2 μM | 606 | <2 | 0 | |||||
| 4 | — | 632 | ND | 0 | ||||
| TNF 2 ng/ml | 1554 | ND | 48 | |||||
| TNF + bFGF 2 ng/ml | 996 | ND | 11 | |||||
| 5 | — | 108 | ND | 1 | ||||
| TNF 5 ng/ml | 348 | ND | 56 | |||||
| TNF + KT5926 5 μM | 126 | ND | 6 | |||||
| UV light 0.08 J/cm2 | 352 | ND | 67 | |||||
| UV light + KT5926 5 μM | 128 | ND | 8 |
*
Cells were pretreated with inhibitors for 1 h except for cpDEVD-CHO and bFGF, which were pretreated for 3 h before exposure to TNF or UV light.
‡
AP24 activity was quantitated using the AAPV-pNa substrate or the nuclear assay after purification of cell lysates on DK120 affinity columns as described in Materials and Methods. ND, not determined.