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. 1997 Oct 6;186(7):1077–1085. doi: 10.1084/jem.186.7.1077

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Effect of IL-8 on IFN-regulated OAS mRNA gene expression. WISH cells were pretreated with medium or IL-8, 4 or 18 h overnight (O/ N), followed by medium or 10 IU/ml IFN-α for 4 h. RNA was reverse transcribed, amplified with primers for OAS and β actin, and gel electrophoresed. Lane designations are as indicated on figure. RT-PCR conditions for OAS gene expression allowed, at least, qualitative comparisons, e.g., significant stimulation or inhibition of signal strength. Identity of OAS fragment was confirmed with oligoprobe specific to OAS (not shown). Experiment is one of three performed.