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. 1997 Oct 6;186(7):1137–1147. doi: 10.1084/jem.186.7.1137

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Quantitation of viable parasite from draining lymph nodes. Single cell suspensions were made from draining lymph nodes harvested from infected mice at various time points after infection. The number of viable parasites was determined from the highest dilution at which promastigotes could be grown out 7 d of incubation. Data shown represents an average of three mice ± one SD at each time point.