Table 3.

TCR-δ Rearrangements from γ_c ⁻ Thymocyte Precursors

DNA source		Vδ4-Jδ1		Vδ5-Jδ1
γc + total thymus		100.00*		100.00
RAG 1−/− thymus		1.61		1.56
γc − total thymus		94.36		75.40
γc − DN sort‡		111.92		55.58
CD3ε Δ5/Δ5 thymus		73.24		101.43

^* Indicated TCR-δ rearrangements were quantitated and expressed as percentage of rearrangements found in DNA isolated from γ_c ⁺ total thymocyte preparations (see Materials and Methods). Results are representative of two independent experiments.  

^‡ Double negative thymocytes (CD4⁻CD8⁻) were sorted from γ_c ⁻ thymi as described in Materials and Methods, and DNA was isolated for analysis.