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VSV8 peptide chaperoned by gp96 is re-presented by macrophage. Pristane-induced peritoneal exudate cells (104) and VSV peptide-specific CTL (5 × 104) were cocultured with gp96– VSV8 complexes (3–20 μg/ml) reconstituted at 37, 60, 85, or 98°C in a 96-well U-bottomed plate at 37°C. After 24 h, supernatants were collected and assayed for TNF-α production in a cytotoxicity assay as described (15).
