Table 3.
Cytokine Secretion by CD8+ T Cell Subsets
Cells* | IL-2 | IFN-γ | TNF-α | IL-4 | ||||
---|---|---|---|---|---|---|---|---|
U/ml | pg/ml | pg/ml | pg/ml | |||||
CD45RA+CD27+ | 100 | 2,860 | 370 | <1 | ||||
CD45RA+CD27− | <10 | 9,310 | 2,220 | <1 | ||||
CD45RA−CD27+ | 100 | 11,940 | 1,660 | 10 |
Sorted cells (2.5 × 104) were stimulated with PMA (100 ng/ml) and ionomycin (1 μM) for 4 d. Cytokine content of the supernatants was tested with a bioassay for IL-2 and ELISAs for IFN-γ, TNF-α, and IL-4.