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. 1997 Nov 3;186(9):1441–1450. doi: 10.1084/jem.186.9.1441

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Thymus and spleen cells from 72ATg and 99ATg mice present exogenous peptides poorly. Thymus and spleen cells were isolated from various types of mice as described in Materials and Methods. They were cultured with different concentrations of Hb and a T cell hybridoma, KH-8.3, specific for IE^k + Hb. The ability of the cells to present the exogenously added peptide was assayed by measurement of production of IL-2 by the T cell hybridoma 24 h later. The results are representative of at least three independent experiments.