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. 1997 Nov 17;186(10):1655–1662. doi: 10.1084/jem.186.10.1655

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In vitro transport of synthetic versions of the 147-155TX constructs. RMA cells were permeabilized with streptolysin O (2 U/ml) and incubated with radioiodinated reporter peptide (amino acid sequence TVNKTERAY) plus the indicated dilutions of test peptides. Reporter peptide transport in TAP-minus RMA-S cells was always assessed as a negative control and typically yielded counts <2% of those seen with RMA + reporter peptide alone. Samples were done singly except for RMA cells with no competitor, done in duplicate. The experiment was repeated four times, all yielding similar results.