Figure 5.

The effect of anti-α₆ and anti-α₄ integrin antibodies on TNF-α–induced epidermal LC migration in vivo. Groups of four mice received 40 μg of (A) anti-α₆ integrin (GoH3) or (B) anti-α₄ integrin (PS/2) antibody intraperitoneally. Controls either received isotype-matched control antibody or were left untreated. 2 h after administration of antibody, two mice per group received 30 μl injection intradermally into both ear pinnae of 50 ng murine TNF-α in 0.1% BSA. The remaining two mice received 30 μl intradermal injection of 0.1% BSA. Ears were removed 30 min later, epidermal sheets were prepared, and the number of LCs/mm² was determined by immunofluorescence analysis. Results are means ± SD (n = 40). Treatment of mice with TNF-α (in both A and B) caused a significant decrease in the frequency of LCs compared with either untreated controls or mice exposed to BSA alone (P <0.005). Treatment with anti-α₆ resulted in a significantly higher frequency of LC compared with mice exposed to TNF-α together with an isotype-matched control antibody (P <0.005; A). In contrast, anti-α₄ failed to affect significantly LC frequency compared with isotype controls.