Skip to main content
. 1997 Nov 17;186(10):1793–1798. doi: 10.1084/jem.186.10.1793

Figure 1.

Figure 1

Figure 1

Figure 1

Tyrosine phosphorylation of Pyk2 in response to chemokines. (a and b) HL60 promyelocytic leukemia cells were incubated with 500 nM RANTES (a) or SDF-1α (b) at 37°C for the indicated times before lysis. (+PTx) Cells were pretreated with pertussis toxin before incubation with chemokines. Pyk2 was immunoprecipitated (IP) with rabbit antibodies against Pyk2 and blotted with antiphosphotyrosine (anti-PTyr) or anti-Pyk2 antibodies. (c) DU6 CD4+ T cells were incubated with RANTES, MIP-1β, or SDF-1α (500 nM each) for the indicated times before lysis and processing as in a and b.