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. 1997 Nov 17;186(10):1793–1798. doi: 10.1084/jem.186.10.1793

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Tyrosine phosphorylation of Pyk2 after contact with HIV-1 envelope glycoprotein on the surface of transfected 293T cells and virions. (a) HL60 or DU6 cells were lysed 30 s after being mixed with 293T cells expressing M-tropic (JRFL), T-tropic (HXB2), or no (−) envelope. (b) DU6 cells were mixed with HIV–luc particles pseudotyped with either JRFL or VSV-G envelopes and lysed after 90 s. As a positive control, MIP-1β was incubated with DU6 cells for 30 s before lysis.

Tyrosine phosphorylation of Pyk2 after contact with HIV-1 envelope glycoprotein on the surface of transfected 293T cells and virions. (a) HL60 or DU6 cells were lysed 30 s after being mixed with 293T cells expressing M-tropic (JRFL), T-tropic (HXB2), or no (−) envelope. (b) DU6 cells were mixed with HIV–luc particles pseudotyped with either JRFL or VSV-G envelopes and lysed after 90 s. As a positive control, MIP-1β was incubated with DU6 cells for 30 s before lysis.