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. 1997 Nov 17;186(10):1793–1798. doi: 10.1084/jem.186.10.1793

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Tyrosine phosphorylation of Pyk2 mediated by T-tropic gp120 can be inhibited by pertussis toxin or a monoclonal antibody against CXCR4. (a) HL60 cells were pretreated with pertussis toxin or left untreated. Treated and untreated cells were incubated with T-tropic gp120 (BH10, 5 μg/ml) for the indicated times before lysis. (b) HL60 cells were resuspended in growth media/0.5% FCS either without antibody (no Ab) or containing monoclonal antibodies specific for CXCR4 (anti-CXCR4) or human CD8 (control Ab). Cells were incubated with antibody at 37°C for 15 min, then mixed with T-tropic SF-2 envelope for 30 s before lysis.

Tyrosine phosphorylation of Pyk2 mediated by T-tropic gp120 can be inhibited by pertussis toxin or a monoclonal antibody against CXCR4. (a) HL60 cells were pretreated with pertussis toxin or left untreated. Treated and untreated cells were incubated with T-tropic gp120 (BH10, 5 μg/ml) for the indicated times before lysis. (b) HL60 cells were resuspended in growth media/0.5% FCS either without antibody (no Ab) or containing monoclonal antibodies specific for CXCR4 (anti-CXCR4) or human CD8 (control Ab). Cells were incubated with antibody at 37°C for 15 min, then mixed with T-tropic SF-2 envelope for 30 s before lysis.