Figure 5.

Phenotype and functional activity of CD8⁺ T cells from RAG-2^−/− versus RAG-2⁺ 8.3-NOD mice. (A) Flow cytometry profiles of CD4⁺CD8⁺ (left) and CD4⁻CD8⁺ thymocytes (right) from 8.3-NOD mice (dotted line) and RAG-2^−/− 8.3-NOD mice (solid line) for maturation markers. Thymocytes were analyzed by three-color cytofluorometry as in Fig. 1. Panels show the fluorescence histograms of each marker on gated CD4⁺CD8⁺ and CD4⁻CD8⁺ thymocytes. (B) Flow cytometry profiles of splenic CD8⁺ T cells from 8.3-NOD mice (dotted line) and RAG-2^−/− 8.3-NOD mice (solid line) for activation and memory markers. (C) Proliferative activity of splenic CD8⁺ T cells from 8.3-NOD mice and RAG-2^−/− 8.3-NOD mice in response to islet cells. (D) Reverse transcription-PCR analysis for cytokine gene expression of islet-derived CD8⁺ T cells from 8.3-NOD mice and RAG-2^−/− 8.3-NOD mice. M, 1Kb ladder. (E) Kinetics of insulitis in RAG-2^−/− 8.3-NOD mice.