Figure 3.

TNF-α induces ceramide generation in intestinal mucosa. (A) Time course of the effect of 25 μg of recombinant human TNF-α/ 25g mouse; (B) dose response at 2 h. These studies were performed as in Fig. 2 except C₅₇BL/6 mice were injected with TNF-α retro-orbitally. The data (mean ± SEM) represent triplicate determinations from two mice per point from one representative of three experiments for A and one representative of four experiments for B.