Figure 3.

The HP-F1γ receptor is an ∼110-kD monomeric glycoprotein. (Left) Immunoprecipitation from ¹²⁵I-labeled NKL cells with HP-F1 mAb yields a protein which runs as a 110-kD band in SDS-PAGE under reducing conditions. After treatment with N-glycosidase, the molecular mass is reduced to ∼90 kD. (Right) Identical electrophoretic patterns were obtained by SDS-PAGE analysis of immunoprecipitates from ILT2-transfected COS7 cells. No bands were detected using the culture supernatant from X63 murine myeloma in control experiments.