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ILT2 is associated with SHP-1. NKL cells and the EBV-transformed B cell line C1R were incubated for 10 min at 37°C either with medium alone or with pervanadate. ILT2 was then immunoprecipitated with the HP-F1 mAb and immunoprecipitates were separated by SDS-PAGE and immunoblotted with anti–SHP-1 antibody. Whole cell lysate is included as a positive control. SHP-1 was recruited to ILT2 after cell stimulation with pervanadate.
