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. 1997 Dec 1;186(11):1911–1922. doi: 10.1084/jem.186.11.1911

Figure 4.

Figure 4

Inhibitors of TCR-CD28–mediated death of DP thymocytes. DP thymocytes from wild-type (B6) mice were stimulated by platebound anti–TCR-β and anti-CD28 in the presence or absence of the following pharmacological agents: the calcineurin inhibitor, cyclosporine A (1 μg/ml); the PI-3-kinase inhibitor, wortmannin (800 ng/ml); the PKCγ inhibitor, GF109203x (800 ng/ml); the protein synthesis inhibitor, cycloheximide (10 μg/ml); and the caspase inhibitor, ZVAD-FMK (100 μM). To compare the effects of various reagents on TCR-CD28–mediated DP thymocyte apoptosis in experiments performed with different solvent controls, individual responses were normalized to their respective controls (killing index). As positive controls for the pharmacologic agents used: cyclosporine A and GF109203x used in this experiment inhibited TCR-mediated CD5 upregulation, and wortmannin used in this experiment blocked NK-mediated target cell lysis (data not shown). Also displayed in the same format are the results of anti–TCR-CD28 stimulation of DP thymocytes isolated from bcl-2 transgenic mice.