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. 1997 Dec 1;186(11):1911–1922. doi: 10.1084/jem.186.11.1911

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A CD28-independent mechanism of TCR-mediated death of DP thymocytes. DP thymocytes from either B6 (Ly 5.1⁺) or CD28 KO (Ly5.1⁺) mice were co-cultured with Ly 5.2⁺ APC in the presence or absence of platebound anti-TCR. Harvested cells were stained with both anti-Ly5.1 antibody and EtBr. DP thymocytes were distinguished from APCs by expression of Ly5.1.