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. 1997 Dec 1;186(11):1923–1931. doi: 10.1084/jem.186.11.1923

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Signal transduction through surface IgM and CD19 in B cells from sHEL/Ig^HEL/hCD19^+/+ (A) or Ig^HEL/hCD19^+/+ mice (B). Relative [Ca⁺⁺]_i levels were assessed by flow cytometry after gating on the B220⁺ population of indo-1 loaded splenocytes. Baseline fluorescence ratios were collected for 1 min before HEL and/or specific monoclonal antibodies were added (arrows) at final concentrations of: HEL, 100 ng/ml; anti-mouse CD19, 40 μg/ml; anti-human CD19, 40 μg/ml. An increase in [Ca⁺⁺]_i over time is shown as an increase in the ratio of indo-1 fluorescence. Values represent the ratios of fluorescence intensity of cell populations after treatment relative to the fluorescence intensity of untreated cells. These results are representative of those obtained from three littermate pairs of mice.