Skip to main content
. 1997 Dec 1;186(11):1923–1931. doi: 10.1084/jem.186.11.1923

Table I.

Phenotype and Frequency of B lymphocytes in Lymphoid Tissues

Tissue Phenotype IgHEL IgHEL hCD19+/+ sHEL IgHEL sHEL IgHEL hCD19+/+
Frequency (%) and number (No. × 10−6) of B cells*
bone % IgM−B220lo      14 ± 2        14 ± 2     13 ± 2       13 ± 2
marrow % IgM+B220lo      32 ± 4        49 ± 10     46 ± 2       53 ± 3
% IgM+B220hi      16 ± 3 8 ± 3§     12 ± 2        8 ± 1
% HSAlo B220hi      25 ± 3         9 ± 3‖     17 ± 5        5 ± 1§
blood % B220+      43 ± 3         7 ± 2‖     22 ± 3        6 ± 2‖
# B220+  2.4 ± 0.5     0.3 ± 0.1‖ 0.9 ± 0.2        0.2 ± 0.1‖
spleen % B220+      36 ± 6        28 ± 5     44 ± 4       17 ± 3‖
# B220+      24 ± 5        14 ± 3§     29 ± 5       15 ± 6§
peritoneum % CD5+ B220lo  2.2 ± 0.3     1.3 ± 0.4 4.5 ± 1.1        1.9 ± 0.7
# CD5+ B220lo  0.05 ± 0.01    0.05 ± 0.02 0.10 ± 0.02   0.04 ± 0.02
% CD5− B220hi      38 ± 6     2.7 ± 0.7‖     14 ± 2  1.2 ± 0.4‖
# CD5− B220hi  1.0 ± 0.3 0.07 ± 0.03§ 0.33 ± 0.03  0.02 ± 0.01‖
Expression Source of B cells Levels relative to IgHEL mice (% ± SEM)‡
IgM levels: bone B220lo 100        69 ± 12‖     41 ± 9       22 ± 8
marrow: B220hi 100        63 ± 4‖ 4.1 ± 0.5        4.3 ± 0.7
blood: B220+ 100        68 ± 15‖     19 ± 3       25 ± 4
spleen: B220+ 100        62 ± 4‖     16 ± 2        7 ± 1‖
I-A levels: blood: IgM+ 100       126 ± 5‖    169 ± 7      254 ± 33‖
spleen: IgM+ 100       259 ± 55§    184 ± 13      283 ± 30§
*

 Cumulative mean (± SEM) frequencies of different cell populations from at least five 2-mo-old mice of each genotype. Flow cytometry gates similar to those shown in Fig. 3 were used to determine the frequency of each cell type within the lymphocyte population. B cell numbers for blood indicate the number of cells/ml. B cell numbers from spleen and peritoneum were determined based on the total number of lymphocytes recovered.  

‡

 Relative cell surface antigen densities were determined by comparing the channel numbers of mean linear fluorescence intensity between IgHEL B cells and B cells fom other mice. Values represent the mean expression levels obtained from at least three sets of mice of each genotype. All samples in each set of mice were stained in parallel and analyzed sequentially by flow cytometry with identical instrument settings.  

§

 Differences between mice not expressing hCD19 and those expressing hCD19 were significant, P <0.05.  

‖

P <0.01.