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. 1997 Dec 15;186(12):2051–2056. doi: 10.1084/jem.186.12.2051

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Reverse transcriptase PCR of LBP mRNA from hemizygous mice and homozygous LBP^−/− littermates. RNA was extracted from F4 mice, and analyzed by reverse transcriptase PCR. Intron spanning primers were used; the DNA product resulting from the reverse transcription and amplification of LBP mRNA was 1.1 kb, whereas contaminating genomic DNA had a predicted size of >10 kb. Shown is one of four representative experiments.