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. 1997 Dec 15;186(12):2051–2056. doi: 10.1084/jem.186.12.2051

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Plasma from the knock-out mice does not contain functional LBP. (a) LPS-coated plastic was incubated with plasma from wild-type LBP^+/+, hemizygous LBP^+/−, and homozygous LBP^−/− mice. LPS-bound LBP was detected by incubating washed wells with polyclonal anti-LBP serum as described in Materials and Methods. No detectable LBP remained bound to the plastic in plasma from the KO animals. (b) Plasma from LBP^+/− animals was compared to plasma from homozygous LBP^−/− animals for its ability to mediate transfer of LPS to sCD14. B-LPS plus LBP^+/− plasma plus sCD14 (•), B-LPS plus LBP^−/− plasma plus sCD14 (▴), B-LPS plus LBP^+/− plasma (○), B-LPS plus LBP^−/− plasma (▵), B-LPS alone (□). The KO plasma had virtually no ability to transfer LPS to sCD14. Addition of recombinant LBP to LBP^−/− plasma restored activity (not shown). Results are representative of four separate experiments.

Plasma from the knock-out mice does not contain functional LBP. (a) LPS-coated plastic was incubated with plasma from wild-type LBP^+/+, hemizygous LBP^+/−, and homozygous LBP^−/− mice. LPS-bound LBP was detected by incubating washed wells with polyclonal anti-LBP serum as described in Materials and Methods. No detectable LBP remained bound to the plastic in plasma from the KO animals. (b) Plasma from LBP^+/− animals was compared to plasma from homozygous LBP^−/− animals for its ability to mediate transfer of LPS to sCD14. B-LPS plus LBP^+/− plasma plus sCD14 (•), B-LPS plus LBP^−/− plasma plus sCD14 (▴), B-LPS plus LBP^+/− plasma (○), B-LPS plus LBP^−/− plasma (▵), B-LPS alone (□). The KO plasma had virtually no ability to transfer LPS to sCD14. Addition of recombinant LBP to LBP^−/− plasma restored activity (not shown). Results are representative of four separate experiments.