Figure 6.

In vivo cytokine response to LPS injection in homozygous −/− KO and hemizygous +/− mice are nearly identical. Age- and sex-matched mice were injected intravenously under anesthesia with 200 μl of LPS at the indicated concentrations via the retroorbital plexus. After 90 min, mice were reanesthetized and phlebotomized using heparinized capillary tubes. Each of three mice was assessed separately per condition. Plasma TNF-α was determined by ELISA. Shown is a representative experiment from six similar experiments (three intravenous and three intraperitoneal injections).