Table 1.
T Cell Populations in the Thymus and Lymph Nodes of +/+, fyn−/−, itk−/−, and fyn−/−itk−/− Mice
| Genotype | Source | Total cells | Percentage T cells§ | Total T cells§ | Percentage of total cells | CD4/CD8 ratio | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CD4−CD8− | CD4+CD8+ | CD4+CD8− | CD4−CD8+ | |||||||||||||||
| 10−7 | 10−7 | |||||||||||||||||
| +/+ (n = 12)* | Thymus | 10.2 ± 4.3 | 2.1 ± 0.8 | 85.7 ± 4.5 | 9.0 ± 3.5 | 3.2 ± 1.3 | 3.0 ± 1.0 | |||||||||||
| fyn −/− (n = 3)* | Thymus | 17.2 ± 3.3 | 2.0 ± 1.2 | 88.6 ± 1.9 | 6.9 ± 2.6 | 2.6 ± 0.3 | 2.6 ± 0.7 | |||||||||||
| itk −/− (n = 16)* | Thymus | 8.7 ± 1.8 | 3.2 ± 1.2 | 80.7 ± 8.0 | 7.1 ± 2.6 | 8.9 ± 6.2 | 1.0 ± 0.5 | |||||||||||
| fyn −/− itk −/− (n = 7)* | Thymus | 12.3 ± 4.2 | 3.7 ± 1.5 | 84.7 ± 4.5 | 6.7 ± 2.8 | 5.0 ± 1.5 | 1.4 ± 0.5 | |||||||||||
| +/+ (n = 12)* | Lymph nodes‡ | 6.1 ± 1.4 | 77.8 ± 8.0 | 4.7 ± 1.1 | 3.3 ± 1.5 | 1.2 ± 0.9 | 47.4 ± 9.2 | 26.0 ± 2.4 | 1.8 ± 0.4 | |||||||||
| fyn −/− (n = 3)* | Lymph nodes‡ | 6.6 ± 1.6 | 74.8 ± 6.9 | 5.0 ± 1.6 | 1.9 ± 1.0 | 1.4 ± 1.9 | 47.0 ± 4.7 | 24.4 ± 3.0 | 2.0 ± 0.3 | |||||||||
| itk −/− (n = 16)* | Lymph nodes‡ | 6.1 ± 2.3 | 60.7 ± 9.2 | 3.6 ± 1.4 | 5.2 ± 2.3 | 1.1 ± 1.9 | 27.2 ± 5.5 | 27.2 ± 7.9 | 1.2 ± 0.7 | |||||||||
| fyn −/− itk −/− (n = 7)* | Lymph nodes‡ | 5.9 ± 2.0 | 54.8 ± 7.6 | 3.2 ± 1.0 | 5.8 ± 3.7 | 0.7 ± 0.4 | 25.7 ± 3.2 | 22.6 ± 5.1 | 1.2 ± 0.4 | |||||||||
Data are expressed as mean ± standard deviation.
*
Mice, 7–12 wk old; numbers of animals studied are shown in parentheses.
‡
Cervical, brachial, axillary, superficial inguinal, and mesenteric.
§
The number of T cells in the lymph nodes was calculated based on the percentages of cells staining positive with anti-CD3ε.