Table 1.

Autoimmune Diabetes in RIP-mOVA Mice Depends on the Number of Adoptively Transferred OT-I Cells

No. of OT-I cells transferred		No. mice diabetic/ total No. mice (%)
10 × 106		3/3		(100%)
5 × 106		18/18		(100%)
2 × 106		9/10		(90%)
1 × 106		4/8		(50%)
0.5 × 106		1/12		(8%)
0.25 × 106		0/26		(0%)
0.10 × 106		0/7		(0%)

Various numbers of Vα2⁺ Vβ5⁺ CD8⁺ cells prepared from OT-I RAG^−/− mice were injected i.v. into unirradiated RIP-mOVA mice between 6 and 8 wk of age. These recipients were monitored daily for glucosuria. Mice that did not develop diabetes were monitored for at least 25 d and 5 recipients of 0.25 × 10⁶ OT-I cells for 210 d. These data have been accumulated over six experiments.