Table 1.
Autoimmune Diabetes in RIP-mOVA Mice Depends on the Number of Adoptively Transferred OT-I Cells
| No. of OT-I cells transferred | No. mice diabetic/ total No. mice (%) | |||
|---|---|---|---|---|
| 10 × 106 | 3/3 | (100%) | ||
| 5 × 106 | 18/18 | (100%) | ||
| 2 × 106 | 9/10 | (90%) | ||
| 1 × 106 | 4/8 | (50%) | ||
| 0.5 × 106 | 1/12 | (8%) | ||
| 0.25 × 106 | 0/26 | (0%) | ||
| 0.10 × 106 | 0/7 | (0%) | ||
Various numbers of Vα2+ Vβ5+ CD8+ cells prepared from OT-I RAG−/− mice were injected i.v. into unirradiated RIP-mOVA mice between 6 and 8 wk of age. These recipients were monitored daily for glucosuria. Mice that did not develop diabetes were monitored for at least 25 d and 5 recipients of 0.25 × 106 OT-I cells for 210 d. These data have been accumulated over six experiments.