Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Dec 15;186(12):2033–2038. doi: 10.1084/jem.186.12.2033

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Antigen recognition, IFN-γ production and TCR-ligand binding for IASA-YIPSAEK(ABA)I (wt) and variants on cloned CTL. The normalized relative antigenic activities (cytotoxicity) and TCR-ligand binding at 26°C are shown as open and full bars, respectively (a), and the IFN-γ production as open bars (b). All values were normalized relative to IASA-YIPSAEK(ABA)I. The cases for which the CTL response was ⩾5-fold lower than TCR-ligand binding are shown in red and those for which it was ⩾5-fold higher in green. TCR antagonists are shown in purple. The variants tested include IASA-YIASAEK(ABA)I (P255A), IASA-YIPSAAK(ABA)I (E258A), IASA-YISSAEK(ABA)I (P255S), IASA-YIPAAEK(ABA)I (S256A), IASA-YILSAEK(ABA)I (P255L), and the CTL clones T1, S14, and S17. Some of the experiments were performed in the presence of SF1-1.1.1 Fab′. Each experiment was performed in triplicates and the mean values and standard deviations were calculated from at least two experiments.

Antigen recognition, IFN-γ production and TCR-ligand binding for IASA-YIPSAEK(ABA)I (wt) and variants on cloned CTL. The normalized relative antigenic activities (cytotoxicity) and TCR-ligand binding at 26°C are shown as open and full bars, respectively (a), and the IFN-γ production as open bars (b). All values were normalized relative to IASA-YIPSAEK(ABA)I. The cases for which the CTL response was ⩾5-fold lower than TCR-ligand binding are shown in red and those for which it was ⩾5-fold higher in green. TCR antagonists are shown in purple. The variants tested include IASA-YIASAEK(ABA)I (P255A), IASA-YIPSAAK(ABA)I (E258A), IASA-YISSAEK(ABA)I (P255S), IASA-YIPAAEK(ABA)I (S256A), IASA-YILSAEK(ABA)I (P255L), and the CTL clones T1, S14, and S17. Some of the experiments were performed in the presence of SF1-1.1.1 Fab′. Each experiment was performed in triplicates and the mean values and standard deviations were calculated from at least two experiments.