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. 1998 Jan 5;187(1):97–104. doi: 10.1084/jem.187.1.97

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Effect of LMP7 active site mutations on LMP2 processing. Proteasomes were immunoprecipitated with MCP21 from lysates of T2 cells transfected with LMP2 and LMP7 (2 + 7), LMP2 and LMP7(T1A) [2 + 7(T1A)], and LMP2 and LMP7(K33A) [2 + 7(K33A)]. Specific subunits were visualized by immunoblotting after SDS-PAGE. Immunoprecipitates from 10⁷ cells were loaded per lane. ppLMP7, partially processed LMP7. Partial loss of the pre-LMP7 band in lane 2 + 7 is due to poor transfer as a result of an air bubble.