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Immunohistochemical detection of cell-associated TSP1 in human gingival mucosa. Fixed oral epithelial tissue thin section was incubated with polyclonal antiserum reactive against both TSP1 and 2 (lower panel), or with preimmune serum (upper panel), followed by biotinylated second antibody, and developed using avidin-conjugated peroxidase. Brown deposits indicate sites of thrombospondin reactivity. Original magnification, ×200.
