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. 1998 Jan 5;187(1):37–48. doi: 10.1084/jem.187.1.37

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Deglycosylation of membrane tyrosinase produces a band which comigrates or nearly comigrates with the tyrosinase species found in the cytosol. Lane 1 contains the membrane fraction from 5 × 10⁵ cells mock treated with PNGase F. Lanes 2–5 contain equivalent DM93 membrane fractions treated with 1.5 mU PNGase for 2, 4, 8 and 12 h, respectively. Lane 6 contains the cytosolic fraction of 5 × 10⁵ DM93 cells treated with lactacystin for 5 h.