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. 2001 Mar 19;152(6):1145–1158. doi: 10.1083/jcb.152.6.1145

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Effect of V12Rac expression in growing Swiss 3T3 cells. Swiss 3T3 cells in growth medium were injected with pRK5–myc-V12Rac (100 μg/ml). Cells were then incubated in the absence or presence of GRGDdSP (RGDd) (1.2 mM), GPenGRGDSPCA (cRGD) (0.05 mM), or hamster anti–mouse β3 integrin (clone 2C9.G2) (20 μg/ml) as indicated for 4 h before fixing and staining. Rac-expressing cells were identified with mouse anti-myc (clone 9E10) followed by FITC goat anti–mouse antibodies, and the number of cells expressing clearly identifiable protrusions was determined. Data are average ± SD for at least three experiments, each examining 100 injected cells. The inset shows the typical morphology of the actin cytoskeleton in a V12Rac-expressing cell. Bar, 10 μm.