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. 2001 Mar 19;152(6):1197–1206. doi: 10.1083/jcb.152.6.1197

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Chemotactic response of RSMC to the HMG box domains of HMG1. (A) Concentration-dependent response to Box A and Box B, both expressed in E. coli. Random cell migration is referred to as 100% migration. The data represent the mean ± SD (n = 3). The statistical significance of the result is P < 0.0001 in an ANOVA model, for both Box A and Box B. (B) Effects of full-length HMG1 expressed in E. coli (100 ng/ml), Box A+B (100 ng/ml), Box A (10 ng/ml), or Box B (10 ng/ml) on actin cytoskeleton organization. Cells were incubated with the indicated molecule for 30 min. Actin filaments were visualized using TRITC-phalloidin.