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. 2001 Mar 19;152(6):1197–1206. doi: 10.1083/jcb.152.6.1197

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Pertussis toxin (PT) inhibits HMG1-induced RSMC migration and actin cytoskeleton reorganization. (A) Chemotaxis assays. The data represent the mean ± SD. The value of 100% corresponds to the number of cells migrating in the absence of any stimulator (random cell migration). (B) Cytoskeleton reorganization. RSMC were pretreated for 6 h either with PT or mPT (both at 50 ng/ml), and were then stimulated (at time 0) with either 100 ng/ml HMG1, 10 ng/ml Box A, or 10 ng/ml Box B. The stimulation with HMG1 was carried on for 30 min. Actin filaments were visualized using TRITC-phalloidin.