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The MAP kinase pathway is involved in HMG1 signaling. RSMC, pretreated or not for 1 h with 50 μM PD98059, were stimulated for 30 min with 100 ng/ml calf thymus HMG1. Cells were then stained with antibodies against phosphorylated ERK1/2 and DAPI. A separate sample of cells were stained with TRITC-phalloidin to visualize the reorganization of the cytoskeleton.
