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. 2001 Mar 19;152(6):1255–1266. doi: 10.1083/jcb.152.6.1255

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Centromere proximal spots exhibited dynamic separation and oscillations along the preanaphase mitotic spindle. The lacO marker was integrated ∼1.1 kb from CEN11 with Spc72–GFP-labeled spindle pole bodies. (A) Selected frames of an ∼7.5-min single-plane time-lapse. (B) A kymograph sequence of the entire time-lapse collected at ∼0.9-s intervals. Narrow tick marks indicate corresponding time points to the above selected frames. (C) Graphical plot of the above time course. In both A and B, arrowheads denote the spindle pole bodies, and arrows define the centromere proximal chromosome spots. Elapsed time provided in seconds. Bar, 2 μm.