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. 2001 Mar 19;152(6):1255–1266. doi: 10.1083/jcb.152.6.1255

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Centromere dynamics of all chromosomes using the pan-specific centromere marker Cse4p fused to GFP (Cse4–GFP). Dynamic movements between the two clustered groups indicated a similar separation and dynamic movements to those observed for the ∼1.1-kb centromere proximal marker. Spindle pole bodies are not labeled. (A) Selected images of an ∼9-min single-plane time-lapse. (B) Kymograph of the entire time-lapse taken at 5-s intervals. Arrowheads in A and B indicate occurrences of GFP centromere fluorescence disjoining from the clustered group. Narrow tick marks indicate corresponding times to A. Elapsed time is in seconds. Bar, 2 μm.