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. 2001 Mar 19;152(6):1247–1254. doi: 10.1083/jcb.152.6.1247

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Angiomotin is expressed in endothelial cells in vivo. (A) Northern blot analysis of angiomotin expression in fetal and adult human tissues. 2 μg of poly A⁺ RNA was analyzed for expression of angiomotin using a 1-kb probe from the 5′ region. Two detectable transcripts were detected in both fetal and adult tissues (multitissue blot from CLONTECH Laboratories, Inc.; H, heart; Br, brain; Pl placenta; Lu, lung; Li, liver; Ki, kidney; Pa, pancreas; M, marker). (B) Western blot analysis using immunoaffinity-purified rabbit polyclonal antibodies against angiomotin. The antibodies detected a band with an approximate molecular mass of 75 kD in angiomotin-transfected NIH 3T3 (Am.) cells but not in the vector (vect.) control. (C) Immunohistochemical localization of ABP-1 protein in human placenta. Paraffin-embedded placental tissue from term placenta was stained with immunoaffinity-purified angiomotin polyclonal antibodies. (C, a) Shows angiomotin protein in endothelium of larger vessels in a placental villus. (C, b) Immunostaining using a monoclonal antibody against CD34, an endothelial marker. (C, c) Detection of angiomotin protein in capillaries of placental microvilli. (C, d) IgG negative control. (D) Expression of angiomotin in a dermal Kaposis sarcoma. (D, a) Shows angiomotin staining of a blood vessel within the Kaposis lesion. (D, b) CD34 staining of a consecutive section of D, panel a. (D, c) Angiomotin-negative dermal blood vessel (arrow) in the normal tissue adjacent to the Kaposis lesion. (D, d) Same as D, panel c, but stained with CD34. D, panels e and f, are negative controls stained with nonimmune rabbit IgG as a negative control. Bars, 40 μm.