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. 2001 Mar 19;152(6):1247–1254. doi: 10.1083/jcb.152.6.1247

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Subcellular localization of transfected and endogenous angiomotin. GFP-tagged angiomotin was expressed in NIH 3T3 cells using a retroviral expression system. Colocalization of GFP-angiomotin (a) with FAK (b) in the extending lamellipodium. (c) Immunofluorescence staining with a polyclonal antibody against angiomotin shows similar localization of endogenous angiomotin in a migrating HUVEC. (d) Control IgG from the same immunized animal. e and f shows the localization of angiomotin (e) to actin ruffles (f) and focal complexes in spreading HUVECs as visualized by phalloidin staining (arrows). Bars, 10 μm.