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. 2001 Mar 19;152(6):1123–1134. doi: 10.1083/jcb.152.6.1123

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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ugo1Δ cells grow slowly on glucose-containing medium and are inviable on nonfermentable carbon sources. (A) 10 meiotic products from the ugo1::HIS3/UGO1 diploid strain, YHS91, were separated by micromanipulation and allowed to grow at 30°C for 5 d on YEPD. (B) 10 tetrads from the sporulated ugo1::HIS3/UGO1 diploid strain, YHS91, were patched onto YEPD medium and then replica-plated to SD medium lacking histidine (SD-His) and YEPGE. Cells were incubated at 30°C for 6 d.

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