Figure 3.

ugo1Δ cells contain fragmented mitochondria and lack mtDNA. Wild-type (FY833), rho⁰ WT (YHS92), and ugo1Δ (YHS72) and fzo1Δ (YHS74) cells were transformed with OM45-GFP–expressing plasmid, pKC2 (Cerveny et al. 2001). Cells were grown to log phase in SRaf medium. Cells were stained using 1 μg/ml DAPI, and viewed by DIC and fluorescence (OM45-GFP or DAPI) microscopy. rho⁰ cells (YHS92) were generated by treating wild-type cells (FY833) with 25 μg/ml ethidium bromide as described (Fox et al. 1991). N, nuclear DNA staining. Bar, 3 μm.