Figure 4.

Morphology and distribution of vacuoles, the endoplasmic reticulum, and the actin cytoskeleton are normal in ugo1Δ cells. (A) Wild-type (FY833, WT) and ugo1Δ (YHS72) cells were grown to log phase in YEPD medium. Cells were then stained with 12 μM FM4-64 (Molecular Probes) to label vacuoles (Vida and Emr 1995) and examined by fluorescence (FM4-64) and DIC microscopy. (B) Wild-type and ugo1Δ cells expressing GFP fused to the COOH terminus of Sec63p from pPS1530 (Prinz et al. 2000) were grown to log phase in SD medium and examined by fluorescence microscopy. (C) Wild-type and ugo1Δ cells were grown to log phase in YEPGal medium. Cells were then fixed in 3.7% formaldehyde and stained with 1 μM Alexa 594–phalloidin (Molecular Probes) to visualize actin filaments (Adams and Pringle 1991). Bars, 3 μm.