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. 2001 Mar 19;152(6):1123–1134. doi: 10.1083/jcb.152.6.1123

Figure 6.

Figure 6

Mitochondrial fusion is defective in ugo1Δ and ugo1Δ dnm1Δ cells. MATa cells containing matrix-targeted RFP under the control of the GAL1 promoter (pHS51) were grown to log phase in SRaf medium and then transferred to SGalSuc medium for 3–5 h to induce the expression of the COX4-RFP fusion protein. MATα cells containing matrix-targeted CFP under the control of the GAL1 promoter (pHS52) were grown to log phase in SGalSuc medium overnight to induce COX-CFP. MATa and α cells were mated for 3.5 h on YEPD medium. The distribution of COX4-RFP and COX4-CFP in representative zygotes containing a medial bud (asterisks) is shown. Zygotes formed by mating between wild-type cells (FY833 and FY834, WT), ugo1Δ mutants (YHS72 and YHS73), dnm1Δ mutants (YHS83 and YHS84), and ugo1Δ dnm1Δ mutants (YHS85 and YHS86) were examined. Bar, 3 μm.

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