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. 2002 Jan 21;156(2):233–240. doi: 10.1083/jcb.200106142

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Copyright © 2002, The Rockefeller University Press

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Figure 5. — TeNT H_C retrograde carriers partially colocalize with NGF-labeled compartments. MNs were incubated with TeNT H_C Alexa488 and Texas red NGF for 30 min at 37°C. Cells were then washed and imaged by time-lapse confocal microscopy. The cell body is located out of view to the right. (a) TeNT H_C Alexa488 and (b) Texas red NGF colocalize in retrograde carriers (arrowhead, asterisk, arrow) (see Video 3, available at http://www.jcb.org/cgi/content/full/200106142/DC1). (c) Merged image of a and b. (d) Corresponding DIC image. TeNT H_C (e) and NGF (f) are found only in round vesicles (arrowhead), whereas tubules appear to be labeled only by TeNT H_C (*). (g) Merged image of e and f. (h–j) Many TeNT H_C carriers colocalize with p75^NTR (arrowheads). MNs were incubated with TeNT H_C Alexa488 (h), fixed, immunostained for p75^NTR (i), and imaged by confocal microscopy. (j) Merged image of h and i. Bars, 5 μm.