Table I. Iodide ion quenching of NBD-labeled integration intermediates in intact and lumen-extracted ER microsomes.
| Intermediate components | Observed Ksv (M− 1) | |
|---|---|---|
| −PFO | +PFO | |
| 111p-86 • KRMa | 0.4 | 1.8 |
| 111p-86 • XRM | 1.7 | 1.8 |
| 111p-86 • XRM + αSec61αb | 0.9 | 1.7 |
| 111p-91 • KRMa | 0.5 | 0.5 |
| 111p-91 • XRM | 2.2 | 2.5 |
| 111p-91 • XRM + αSec61αb | 1.3 | 2.8 |
| 111p-93 • KRMa | 3.6 | 3.7 |
| 111p-93 • XRM | 3.0 | 2.8 |
The Ksv values shown are the average of 3–5 independent experiments except where indicated. The standard deviations for the Ksv values were: ±0.2 M−1 for KRM measurements; ±0.1–0.3 M−1 for XRM – PFO measurements; and ±0.1–0.4 M−1 for XRM + PFO measurements. Samples were prepared as described in Materials and methods.
a
Taken from Liao et al. (1997).
b
The Ksv values are the average of two independent experiments.