Table II. Iodide ion quenching of NBD-labeled integration intermediates in ER microsomes reconstituted with rBIP.
| Intermediate components | Observed Ksv (M− 1) | |
|---|---|---|
| −PFO | +PFO | |
| 111p-86 • RRM | 0.4 | 2.0 |
| 111p-86 • RRM + αBiP | 1.8 | 2.0 |
| 111p-86 • RRM + AMPPNP | 2.0 | 2.0 |
| 111p-91 • RRM | 0.9 | 0.8 |
| 111p-91 • RRM + αBiP | 2.4 | 2.4 |
| 111p-91 • RRM + AMPPNP | 2.5 | 2.6 |
| 111p-93 • RRM | 2.6 | 3.1 |
The Ksv values shown are the average of 3–4 independent experiments. The standard deviations were ±0.2 M−1 for RRM – PFO measurements, and between 0.2 and 0.4 M−1 for all other measurements. Samples were prepared as described in Materials and methods.