Table III.
NAD + quenching of NBD-labeled translocation and integration intermediates in lumen-extracted ER microsomes
| Intermediate components | Observed Ksv (M− 1) | |
|---|---|---|
| −PFO | +PFO | |
| pPL-sK-78 • XRM | 0.4 | 1.8 |
| 111p-86 • XRM | 0.0 | 0.1 |
| 111p-91 • XRM | 0.5 | 0.5 |
| 111p-93 • XRM | 1.8 | 1.7 |
The Ksv values shown are the average of 2–3 independent experiments. The standard deviations were between 0.1 and 0.4 M−1 for all measurements. Samples were prepared as described in Materials and methods. The modified pPL construct, pPL-sK-78, is described in Crowley et al. (1994).