Figure 7.
Clustering of Nav1.6 at NR of βIV-spectrin –null sciatic nerves. Double staining of wild-type (A, A′, and A′′) and βIV-spectrin–null (B, B′, and B′′) sciatic nerves with anti–βIV-spectrin (A and B) and anti-Nav1.6 (A′ and B′) antibodies. (A′′) and (B′′) are merged images. Arrowheads in A′, B′, A′′, and B′′ indicate some of the Nav1.6-positive NR which are also positive for βIV-spectrin in the wild-type (A). Insets in A′ and B′ show enlarged images of typical NR. In C, the number of Nav1.6-positive NR in wild-type (+/+) and βIV-spectrin–null (−/−) sciatic nerve sections were quantified. Mean numbers per 0.1 mm2 field are shown with SD (n = 9 for each genotype). Mice were killed at 3 mo of age. Bars, 100 μm.